Mineralization by mesenchymal stromal cells is variously modulated depending on commercial platelet lysate preparations

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MESENCHYMAL STROMAL CELLS IMPLANTATION IN COMBINATION WITH PLATELET LYSATE PRODUCT IS SAFE FOR RECONSTRUCTION OF HUMAN LONG BONE NONUNION

This was Presented in 5th International Congress of Iranian Iranian Society of Knee Surgery, Arthroscopy, and Sports Traumatology (ISKAST), 14-17 Feb 2018- Kish, Iran

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mesenchymal stromal cells implantation in combination with platelet lysate product is safe for reconstruction of human long bone non:union:

objective: non:union: is defined as a minimum of 9 months since injury without any visible progressive signs of healing for 3 months. recent literature has shown that the application of mesenchymal stromal cells is safe, in vitro and in vivo, for treating long bone non:union:. the present study was performed to investigate the safety of mesenchymal stromal cell (msc) implantation in combination...

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Mesenchymal Stromal Cells Implantation in Combination with Platelet Lysate Product Is Safe for Reconstruction of Human Long Bone Nonunion

OBJECTIVE Nonunion is defined as a minimum of 9 months since injury without any visible progressive signs of healing for 3 months. Recent literature has shown that the application of mesenchymal stromal cells is safe, in vitro and in vivo, for treating long bone nonunion. The present study was performed to investigate the safety of mesenchymal stromal cell (MSC) implantation in combination with...

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Effects of Platelet Lysate Preparations on the Proliferation of HaCaT Cells

BACKGROUND Standard protocols are lacking for the preparation of platelet lysates (PL) as an alternative to using fetal bovine serum as a cell culture supplement. This study aimed to establish optimum conditions for preparing PL for use in cell cultures. METHODS Cell density in three pooled platelet concentrates (PC) were adjusted to 1×10(12)/L and 2×10(12)/L. PL was prepared from PC by 1 to ...

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Platelet Lysate: The Better Choice for Jaw Periosteal Cell Mineralization

Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory. In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in comparison to that of FCS-cultured JPCs. By cell impedance measurements, we det...

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ژورنال

عنوان ژورنال: Cytotherapy

سال: 2018

ISSN: 1465-3249

DOI: 10.1016/j.jcyt.2017.11.011